Tm计算

本工具用于计算引物Tm值，提供三种算法：SantaLucia[1]和Sugimoto[2]的方法采用经典的NN邻近原则，通过热力学参数计算Tm，适合计算20bp以内的引物，Ahsen[3]的算法考虑了离子、GC含量等因素，适合预测20-30bp的引物Tm。

请输入oligo序列:

请输入K⁺浓度（mmol/L）:

请输入Mg²⁺浓度（mmol/L）:

请输入dNTP浓度（mmol/L）:

请输入引物浓度（μmol/L）:

[1] SantaLucia,, J., Allawi, H. T., & Seneviratne, P. A. (1996). Improved Nearest-Neighbor Parameters for Predicting DNA Duplex Stability†. Biochemistry, 35(11), 3555–3562.

[2] Sugimoto, N., Nakano, S. -i., Yoneyama, M., & Honda, K. -i. (1996). Improved Thermodynamic Parameters and Helix Initiation Factor to Predict Stability of DNA Duplexes. Nucleic Acids Research, 24(22), 4501–4505.

[3] von Ahsen N, Wittwer C T, Schütz E. Oligonucleotide melting temperatures under PCR conditions: nearest-neighbor corrections for Mg2+, deoxynucleotide triphosphate, and dimethyl sulfoxide concentrations with comparison to alternative empirical formulas[J]. Clinical Chemistry, 2001, 47(11): 1956-1961.